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Part:BBa_K1088016:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-28)


HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a scarsite between the RBS of araC (BBa_B0030) aswell as in front of and behind the RBS of the prenyltransferase to improve translational efficieny. There are no more scarsites because the rest is assembled with USER cloning


Source

Apart from the prenyltransferase all the parts derive from E. coli. The prenyltransferase have been synthesized based on the cDNA sequence of a Hevea brasiliensis latex extract study. For more info, look at BBa_K1088003.

References